Kuki Polyacrylamide ikoreshwa kuri protein electrophorei

Mu rwego rwa siyanse igezweho, poroteyine electrophoreis ihagaze nkubuhanga bwibanze bwo gusesengura no kuranga poroteyine.Intandaro yubu buryo bukoreshwaPolyacrylamide, ibice byinshi bikora nkumugongo wa matrices ya gel ikoreshwa muri sisitemu ya gel electrophorei.Imiterere yihariye ya Polyacrylamide ituma iba igikoresho cyingirakamaro kubashakashatsi nabahanga bashaka kumenya ibibazo bya poroteyine n’imikoranire yabyo.

Polyacrylamide, bakunze kwita PAM, ni polymer synthique ikozwe muri monomers acrylamide.Ubwinshi bwayo butangaje bwatewe nubushobozi bwayo bwo gukora iminyururu ndende, bikavamo ibintu bimeze nka gel bishobora kwakira ubunini butandukanye bwa molekile.Uyu mutungo utuma polyacrylamide umukandida mwiza wo gukora matrices yuzuye ikoreshwa muri protein electrophorei.

Poroteyine electrophorei ni tekinike itandukanya poroteyine ukurikije ubwinshi bwazo n'ubunini.Mugukurikiza urugero rwa poroteyine kumurima wamashanyarazi muri materix ya polyacrylamide, proteyine zimuka zinyuze muri gel ku gipimo gitandukanye, bikavamo imirongo itandukanye ishobora gusesengurwa no kugereranywa.Uku gutandukana gutanga ubushishozi bwingirakamaro kuri protein, kugena uburemere bwa molekile, no kuba isoforms.

Uruhare rwa Polyacrylamide muri Protein Electrophoresis

Guhitamo polyacrylamide kuri proteine ​​electrophoreis yashinze imizi muri kamere yayo ihinduka.Abahanga barashobora guhindura ubunini bwa matrix ya gel kugirango bemere poroteyine zingana.Ubunini bwinshi butanga matrices ikwiranye no gukemura poroteyine ntoya, mugihe intumbero yo hasi ikoreshwa kuri poroteyine nini.Uku guhuza n'imihindagurikire yerekana ko abashakashatsi bashobora guhuza ubushakashatsi bwabo kugira ngo bagere ku gutandukana no gusesengura neza.

Polyacrylamide nka aFlocculant

Imikorere ya Polyacrylamide irenze uruhare rwayo muri gel electrophorei.Irasanga kandi porogaramu nka flocculant mu nganda zitandukanye, nko gutunganya amazi no gucunga amazi mabi.Nka flocculant, polyacrylamide ifasha mugukusanya uduce twahagaritswe mumazi, kuborohereza.Ibi biranga byerekana ubushobozi butandukanye hamwe ningaruka nini kuri siyanse n'inganda.

Iterambere muri Polyacrylamide-ishingiye kuri Electrophoresis

Imyaka yashize yiboneye iterambere ryiterambere muri polyacrylamide ishingiye kuri tekinoroji ya electrophoreis.URUPAPURO Kavukire, SDS-PAGE, hamwe na elegitoroniki ya elegitoroniki ya elegitoronike ni ingero nkeya zerekana uburyo imiterere ya polyacrylamide ihindagurika yatumye habaho uburyo bwihariye bwo gusesengura imiterere ya poroteyine, guhindura nyuma y’ubuhinduzi, n’imikoranire.Ubu buhanga ni ingirakamaro mu bushakashatsi bwa proteomics hamwe nimbaraga zo kuvumbura ibiyobyabwenge.

Mu rwego rwo gusesengura poroteyine, polyacrylamide igaragara nkinshuti ikomeye, ifasha abashakashatsi gucengera mu isi igoye ya poroteyine.Uruhare rwarwo nk'ishingiro rya matrices ya gel muri sisitemu ya electrophoreis ntishobora kuvugwa.Kuva uburyo bwo kuvumbura uburyo bwindwara kugeza guteza imbere imiti mishya, amashanyarazi ya polyacrylamide akomeje gukora siyanse.Mugihe ikoranabuhanga rigenda ritera imbere, ibi bitangaza bya synthique birashoboka ko bizagenda bihinduka, bikarushaho kunonosora imyumvire yacu kuri poroteyine n'imikorere yabyo itabarika.