Kuki Polyacrylamide ikoreshwa muri electrophoresis ya poroteyine

Mu rwego rwa siyansi ya none, electrophoresis ya poroteyine ni uburyo bw'ingenzi bwo gusesengura no kugaragaza poroteyine. Ishingiro ry'ubu buryo niPolyacrylamide, ikintu gifite ubushobozi bwinshi kandi gikoreshwa nk'inkingi ya gel matrices ikoreshwa muri sisitemu ya gel electrophoresis. Imiterere yihariye ya Polyacrylamide ituma iba igikoresho cy'ingenzi ku bashakashatsi n'abahanga mu bya siyansi bashaka gusesengura ingorane za poroteyine n'imikoranire yazo.

Polyacrylamide, ikunze kwitwa PAM, ni polymer ikoze mu buryo bwa synthetic ikozwe muri acrylamide monomers. Ifite ubushobozi bwo gukora iminyururu miremire, bigatuma habaho ikintu gisa na gel gishobora kwakira ingano zitandukanye za molekile. Iyi miterere ituma polyacrylamide iba nziza cyane mu gukora matrices ifite imyenge ikoreshwa muri electrophoresis ya poroteyine.

Electrophoresis ya poroteyine ni uburyo butandukanya poroteyine hashingiwe ku muvuduko wazo n'ingano yazo. Mu gushyira icyitegererezo cya poroteyine mu murima w'amashanyarazi muri matrix ya gel ya polyacrylamide, poroteyine zinyura muri gel ku gipimo gitandukanye, bigatuma habaho imirongo itandukanye ishobora gusesengura no gupimwa. Uku gutandukanya gutanga ubumenyi bw'ingenzi ku buziranenge bwa poroteyine, kumenya uburemere bwa molekile, no kuba hari isoforms.

Uruhare rwa Polyacrylamide muri Electrophoresis ya Poroteyine

Guhitamo polyacrylamide muri poroteyine electrophoresis bishingiye ku buryo ishobora guhinduka. Abahanga mu bya siyansi bashobora guhindura ingano ya gel matrix kugira ngo ihuze na poroteyine z'ingano zitandukanye. Ingano nyinshi zituma habaho imiterere ikomeye ikwiriye gukemura poroteyine nto, mu gihe ingano nke ikoreshwa kuri poroteyine nini. Uku guhinduka kwemeza ko abashakashatsi bashobora guhindura igerageza ryabo kugira ngo bagere ku gutandukanya no gusesengura neza.

Polyacrylamide nk'umutiFlocculant

Akamaro ka Polyacrylamide ntikagarukira ku ruhare rwayo muri electrophoresis ya gel. Iboneka kandi nk'umuti wo gukurura amazi mu nganda zitandukanye, nko gutunganya amazi no gucunga amazi yanduye. Nk'umuti wo gukurura amazi, polyacrylamide ifasha mu guteranya uduce duto two mu mazi, bityo bigatuma tuvanwamo neza. Iyi miterere igaragaza ubushobozi butandukanye bw'iki kinyabutabire n'ingaruka zacyo ku bumenyi n'inganda.

Iterambere muri Electrophoresis ishingiye kuri Polyacrylamide

Imyaka ishize yagaragaye ko hari iterambere rikomeje mu buhanga bwa electrophoresis bushingiye kuri polyacrylamide. PAGE y’umwimerere, SDS-PAGE, na electrophoresis ya gel ifite imiterere ibiri ni ingero nke z’uburyo ubushobozi bwa polyacrylamide bwo guhuza ibintu bwatumye habaho uburyo bwihariye bwo gusesengura imiterere ya poroteyine, impinduka nyuma yo guhindura imiterere y’umubiri, n’imikoranire. Ubu buryo ni ingenzi cyane mu bushakashatsi bwa proteomics no mu bikorwa byo kuvumbura imiti.

Mu rwego rwo gusesengura poroteyine, polyacrylamide igaragara nk'umufatanyabikorwa ukomeye, bigatuma abashakashatsi bashobora gushakisha mu isi y'urusobe rwa poroteyine. Uruhare rwayo nk'ishingiro rya matrices ya gel muri sisitemu ya electrophoresis ntirushobora gukabya. Kuva ku kuvumbura uburyo bw'indwara kugeza ku guteza imbere uburyo bushya bwo kuvura, electrophoresis ishingiye kuri polyacrylamide ikomeje kugira uruhare mu iterambere rya siyansi. Uko ikoranabuhanga rigenda ritera imbere, iki gitangaza cy’ubukorano gishobora gutera imbere, kikarushaho kunoza ubumenyi bwacu kuri poroteyine n'imikorere yazo myinshi.