Kuki polyacrylamide ikoreshwa kuri electrorephosi

Mubice bya siyansi igezweho, poroteyine electrophosis ihagaze nka tekinike ifumbire yo gusesengura no kuranga poroteyine. Kumutima wubu buryo bubeshyaPolyacrylalide, urugo rutandukanye rukora nk'ikigo cya Mal Matride ikoreshwa muri sisitemu ya Gel electrophoresis. Umutungo wihariye wa Polyacrylaside ugira igikoresho cyingenzi kubashakashatsi nabahanga bashaka gutangaza porotexine za poroteyine n'imikoranire yabo.

Polyacrylaside, akenshi yitwa Pam, ni polymer ya sintetike ikozwe muri monomer ya acrylamide. Ibisobanuro byayo bidasanzwe byitirirwa ubushobozi bwayo bwo gukora iminyururu ndende, bikaviramo ibintu bisa na gel bishobora kwakira ingano zitandukanye za molekile zitandukanye. Uyu mutungo utuma polyacrylamide umukandida mwiza wo gukora imirongo myiza ikoreshwa muri porotephoresis.

Proteine ​​electrophoresis ni tekinike itandukanya poroteyine zishingiye kubirego byabo nubunini. Mugukurikiza urugero rwa poroteyine kumurima wamashanyarazi muri polyacrylamide, poroteyine yimuka binyuze kuri gel ku kage gatandukanye, bikavamo amatsinda atandukanye ashobora gusesengurwa no kubarwa. Uku gutandukana gutanga ubushishozi bwingirakamaro muburambanyi bwa proteyine, kwiyemeza kwibigeraho uburemere bwa molecular, no kuboneka.

Uruhare rwa polyacrylamide muri poroteyine electrophores

Guhitamo Polyacrylalide kuri poroteyine electrophosis yashinze imizi muri kamere yayo. Abahanga barashobora guhindura ibintu bya Matrix ya Gel kugirango bakire poroteyine yubunini butandukanye. Ibitekerezo Byinshi bitera matrike bikwiranye no gukemura poroteyine nto, mugihe ibitekerezo byo hasi bikoreshwa mubintu binini. Ubu buryo bwo guhuza amakuru bwemeza ko abashakashatsi bashobora kudoda ubushakashatsi bwabo kugirango bagere ku gutandukana no gusesengura.

Polyacrylamide nka aFlocculant

Polyacrylamide yingirakamaro igera ku ruhare rwayo muri Gel electrophoresis. Irabona kandi ibyifuzo nk'akagari mu nganda zitandukanye, nko gutunganya amazi no gucunga amazi. Nkumukunzi wawe, ifasha za polyacrylalide muri rusange yahagaritswe ibice mumazi, byorohereza kuvanaho. Ibi biranga byerekana ubushobozi butandukanye bwimiterere nubushobozi bukomeye kuri siyansi ninganda.

Iterambere muri Polyacrylalimide ishingiye kuri electrophosis

Imyaka yashize yiboneye iterambere ryiterambere rya polyacrylalimide ishingiye kuri electroprylalimide ishingiye. Urupapuro rwavukire, urupapuro rwa SDS, na gel ebyiri electrophoresi ni ingero nkeya zuburyo bwo guhuza nuburyo bwihariye bwo gusesengura poroteyine, nyuma-amakoraniro. Ubu buhanga ni ntagereranywa mubushakashatsi hamwe nubushakashatsi bwibiyobyabwenge.

Mu kice cy'isesengura rya poroteyine, Polyacrylamide agaragara nka mugenzi wawe w'iterabwoba, ashoboza abashakashatsi kugira ngo basibe isi ikomeye ya poroteyine. Uruhare rwarwo nkishingiro rya matrices muri sisitemu ya electrophores ntirushobora gutera imbere. Kubera uburyo bwo gutanga uburwayi bwo guteza imbere uburyo bwo gushushanya, polyacrylamide bushingiye kuri electroresis bukomeje guhindura iterambere ryubumenyi. Mugihe ubuhangana nikoranabuhanga, ibitangaza bya sintetike birashoboka ko bizagenda neza, byongera gukungahaza ko dusobanukirwa na poroteyine nibikorwa byabo bya Mayriad.